良好的精液质量及精子活力是成功受精的重要保障。在男性因素不育的患者中,精子功能障碍是重要的原因之一,如少精子症、弱精子症、精子顶体反应缺陷等,这样的夫妇不能正常受精,只能采取辅助生殖技术进行治疗。辅助生殖技术在男性不育治疗中的应用,促进了体外提高精子运动和受精能力的研究。许多物质像血清、腹腔液、卵泡液和其他的化学修饰药物如孕酮、腺苷类似物和甲基黄嘌呤等都被用来提高人精子的运动和受精能力。 1 PF与精子活动力 2 PF与精子获能和顶体反应 这些实验差别可能是由多种因素造成的。精子制备方法、精液质量差异、培养基成分、PF处理前是否经过体外获能培养、PF作用时间和浓度、评价精子顶体反应的方法等都可能造成实验结果的差异。 3 PF与精子超激活运动 4 PF与受精 5 PF的临床应用 参考文献: [1] Huang P T,Chen S U,Chao K H,et al. Effects of fertilization promoting peptide,adenosine,and pentoxifylline on thawed human sperm[J]. Arch Androl, 2003,49(2):145153. [2] Breitbart H,Spungin B. The biochemistry of the acrosome reaction[J]. Mol Human Repod, 1997,3:195202. [3] Yunes R,Fernandez P,Doncel G F,et al. Cyclic nucleotide phosphodiesterase inhibition increases tyrosine phosphorylation and hypermotility in normal and pathological human spermatozoa[J]. Biocell, 2005,29(3):28793. [4] Mariappa D,Siva A B,Shivaji S,et al. TyrphostinA47 inhibitable tyrosine phosphorylation of flagellar proteins is associated with distinct alteration of motility pattern in hamster spermatozoa[J]. Mol Reprod Dev, 2006,73(2):215225. [5] Kinutani M. Effects of pentoxifylline on sperm motion characteristics in normozoospermic men defined by a computeraided sperm analysis[J]. Hiroshima J Med Sci, 1999,48(4):117121. [6] Nassar A,Morshedi M,Mahony M,et al. Pentoxifylline stimulates various sperm motion parameters and cervical mucus penetrability in patients with asthenozoospermia[J]. Andrologia, 1999,31(1):915. [7] Nassar A,Mahony M,Morshedi M,et al. Modulation of sperm tail protein tyrosine phosphorylation by pentoxifylline and its correlation with hyperactivated motility[J].Fertil Steril, 1999,71(5):919923. [8] Tarlatzis B C,Kolibianakis E M,Bontis J,et al. Effect of pentoxifylline on human sperm motility and fertilizing capacity[J]. Arch Androl, 1995,34(1):3342. [9] Sharma R K,Kohn S,Padron O F,et al. Effect of artificial stimulants on cryopreserved spermatozoa from cancer patients[J]. J Urol, 1997,157(2):521524. [10] Kolon T F,Philips K A,Buch J P. Pentoxifylline enhancement of postthaw motility in cryopreserved semen of spinal cordinjured men[J]. Int J Fertil Menopausal Stud, 1995,40(3):156160. [11] Terriou P,Hans E,Giorgetti C,et al. Pentoxifylline initiates motility in spontaneously immotile epididymal and testicular spermatozoa and allows normal fertilization,pregnancy,and birth after intracytoplasmic sperm injection[J]. J Assist Reprod Genet, 2000,17(4):194199. [12] Sharma R K,Tolentino M V Jr,Thomas A J Jr,et al. Optimal dose and duration of exposure to artificial stimulants in cryopreserved human spermatozoa[J]. J Urol, 1996,155(2):568573. [13] Kay V J,Coutts J R,Robertson L. Effects of pentoxifylline and progesterone on human sperm capacitation and acrosome reaction[J]. Hum Reprod, 1994,9(12):23182323. [14] Seshagiri P B,Thomas M,Sreekumar A,et al. Pentoxifylline induced signalling events during capacitation of hamster spermatozoa: significance of protein tyrosine phosphorylation[J]. Cell Mol Biol, 2003,49(3):371380. [15] Nassar A,Mahony M,Blackmore P,et al. Increase of intracellular calcium is not a cause of pentoxifyllineinduced hyperactivated motility or acrosome reaction in human sperm[J]. Fertil Steril, 1998,69(4):748754. [16] Esteves S C,Sharma R K,Thomas A J Jr,et al. Cryopreservation of human spermatozoa with pentoxifylline improves the postthaw agonistinduced acrosome reaction rate[J]. Hum Reprod, 1998,13(12):33843389. [17] Bajpai M,Doncel G F. Involvement of tyrosine kinase and cAMPdependent kinase crosstalk in the regulation of human sperm motility[J]. Reproduction, 2003,126(2):183195. [18] Yanagimachi R. Mammalian fertilization[M]// Knobil E,Neill J D. The physiology of reproduction. 2nd. New York:Raven Press Ltd,1994:189317. [19] Calogero A E,Fishel S,Hall J,et al. Correlation between intracellular cAMP content,kinematic parameters and hyperactivation of human spermatozoa after incubation with pentoxifylline[J]. Hum Reprod, 1998,13(4):911915. [20] Yogev L,Gamzu R,Botchan A,et al. Zona pellucida binding improvement effect of different sperm preparation techniques is not related to changes in sperm motility characterizations[J]. Fertil Steril, 2000,73(6):11201125. [21] Yogev L,Homonnai Z T,Gamzu R,et al. The use of hemizona assay in the evaluation of the optimal sperm preparation technique[J]. Hum Reprod, 1995,10(4):851854. [22] Burger M,Sikka S C,Bivalacqua T J,et al. The effect of sildenafil on human sperm motion and function from normal and infertile men[J]. Int J Impot Res, 2000,12(4):229234. [23] Rizk B,Fountain S,Avery S,et al. Successful use of pentoxifylline in malefactor infertility and previous failure of in vitro fertilization: a prospective randomized study[J]. J Assist Reprod Genet, 1995,12(10):710714. [24] Edirisinghe W R,Junk S,Yovich J M,et al. Sperm stimulants can improve fertilization rates in malefactor cases undergoing IVF to the same extent as micromanipulation by partial zona dissection(PZD) or subzonal sperm insemination(SUZI): a randomized controlled study[J]. J Assist Reprod Genet. 1995,12(5):312318. [25] Tournaye H,Devroey P,Camus M,et al. Use of pentoxifylline in assisted reproductive technology[J]. Hum Repr, 1995,10(Suppl 1):7279. [26] Dimitriadou F,Rizos D,Mantzavinos T,et al. The effect of pentoxifylline on sperm motility,oocyte fertilization,embryo quality,and pregnancy outcome in an in vitro fertilization program[J]. Fertil Steril, 1995,63(4):880886. [27] Negri P,Grechi E,Tomasi A,et al. Effectiveness of pentoxifylline in semen preparation for intrauterine insemination[J]. Hum Reprod, 1996,11(6):12361239. [28] Griveau J F, Lobel B, Laurent M C, et al. Interest of pentoxifylline in ICSI with frozenthawed testicular spermatozoa from patients with nonobstructive azoospermia[J]. Reprod Biomed Online.2006,12(1):1418.
|
己酮可可碱对精子运动能力和受精能力的影响简介:
良好的精液质量及精子活力是成功受精的重要保障。在男性因素不育的患者中,精子功能障碍是重要的原因之一,如少精子症、弱精子症、精子顶体反应缺陷等,这样的夫妇不能正常受精,只能采取辅助生殖技术进行 ... 责任编辑:admin
|
最新文章更多推荐文章更多热点文章更多 |