摘 要:本研究探讨Propentofylline对中性粒细胞的调节作用及其它的作用机制。采用鲁米诺依赖的全血化学发光法测定fMLP激活的中性粒细胞产生过氧化氢。结果显示Propentofylline以浓度依赖形式抑制中性粒细胞产生过氧化氢,并增强腺苷对中性粒细胞的抑制作用,但不影响NECA对中性粒细胞的抑制作用。结果提示,Propentofylline通过抑制细胞对腺苷的摄取,增加局部腺苷水平,增强腺苷对中性粒细胞的抑制作用。这种作用也可能是Propentofylline保护心脑组织损伤的机制之一。 Abstract:In this study, we tested the effects and mechanisms of propentofylline on human neutrophils. The neutrophils were stimulated by fMLP (formyl-MetLeuPhe) and hydrogen peroxide was determined by the luminol amplified chemiluminescence. Results showned that propentofylline inhibited the hydrogen peroxide production by neutrophils in concentration-dependent manner. The inhibitory effect of adenosine on fMLP-stimulated neutrophil hydrogen peroxide was markedly increased in the presence of propentofylline. But that of NECA (5`-N-ethylcarboxamidoadenosine) was unaffected, indicated that the inhibitory effect of propentofylline on neutrophils was mediated by inhibiting adenosine uptake and increasing the local concentration of adenosine. This effect could be a mechanism of the protective effects of propentofylline on the ischemia of brain and heart. 腺苷和NECA均抑制中性粒细胞产生过氧化氢。并随着浓度的增加其抑制作用增强。低浓度的Propentofylline (1μmol.L-1)不影响腺苷对中性粒细胞的作用(数据未显示),而高浓度的Propentofylline (100μmol.L-1)明显增强腺苷对中性粒细胞的抑制作用P<0.05,最大抑制率由66%升到81%。然而,NECA对中性粒细胞的抑制作用不受Propentofylline 的影响p>0.05(见表2)。 Table2.The effects of adenosine and NEDA on hydrogen proxide production in the presence 讨论 组织缺血激活中性粒细胞产生大量氧自由基,与细胞膜上的脂质结合,引起细胞膜功能失调,导致细胞内钙超载,细胞死亡[4]。缺血也刺激腺苷的产生,现已证实,腺苷抑制中性粒细胞粘附到内皮细胞,降低氧自由基释放。血管内皮细胞通过释放腺苷阻止激活白细胞对内皮细胞的损伤[5]。然而,由于外源性腺苷对心血管系统严重的副作用,如低血压和窦性心动过缓,临床应用受到限制。近年来研究趋于促进内源性腺苷的产生或抑制细胞对腺苷的摄取,利用内源性腺苷发挥作用。 |
Propentofylline-对中性粒细胞的调节作用简介:
摘 要:本研究探讨Propentofylline对中性粒细胞的调节作用及其它的作用机制。采用鲁米诺依赖的全血化学发光法测定fMLP激活的中性粒细胞产生过氧化氢。结果显示Propentofylline以浓度依赖形式抑制中性粒 ... 责任编辑:admin
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